Last edited by Mektilar
Friday, May 8, 2020 | History

4 edition of Oligonucleotide Synthesis found in the catalog.

Oligonucleotide Synthesis

M. J. Gait

Oligonucleotide Synthesis

A Practical Approach

by M. J. Gait

  • 340 Want to read
  • 38 Currently reading

Published by Oxford University Press, USA .
Written in English

    Subjects:
  • Organic chemistry,
  • Synthesis,
  • Nucleotides,
  • Organic Synthesis,
  • Psychology,
  • Science,
  • General,
  • Science / Cytology,
  • Nucleic acids,
  • DNA

  • The Physical Object
    FormatPaperback
    Number of Pages232
    ID Numbers
    Open LibraryOL8290045M
    ISBN 100904147746
    ISBN 109780904147742

    ISBN: OCLC Number: Description: xiii, pages: illustrations ; 23 cm. Contents: An introduction to modern methods of DNA synthesis / Michael J. Gait --Preparation of protected deoxyribonucleotides / Roger A. Jones --Solid phase synthesis of oligodeoxyribonucleotides by the phosphite-triester method / Tom Atkinson and Michael Smith --Solid-phase synthesis. Dr. Monia has published more than primary research manuscripts, reviews and book chapters, and is an inventor on more than issued patents. He serves as a senior editor for the journal Nucleic Acid Therapeutics, is on the board of directors for Dynacure, and has served as president of the Oligonucleotide Therapeutics Society (OTS). Dr.

    Prior to oligo synthesis, each solid support is fixed with a phosphoramidite monomer—a chemically-modified DNA nucleotide (Figure 1). The modified nucleotide is covalently attached to the solid support through its 3′ carbon. As oligonucleotide synthesis proceeds in the 3′→5′ direction, it becomes the 3′-most nucleotide of the oligo. of the international gurus of oligonucleotide chemistry to produce a volume which is both comprehensive and authorita- tive. The book, with 19 chapters, spans a wide range of complexity. Some of the chapters, or parts thereof (such as: “Oligodeoxyribonucleotide synthesis: phosphoramiditeAuthor: David M. Tidd.

    Techniques for oligonucleotide synthesis: Solution phase synthesis: In this method of synthesis all reactions are carried out in homogeneous solutions (Chaudhari , ). The first step of condensation is followed by deprotection at the 5 1 -end. TheFile Size: 2MB.   Reverse DNA oligonucleotide synthesis (i.e. from 5′→3′) is a strategy that has yet to be exploited fully. While utilized previously for the construction of alternating 3′-3′- and 5′-5′-linked antisense oligonucleotides, the use of nucleoside 5′-phosphoramidites has not generally been used for the elaboration of (modified) by:


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Oligonucleotide Synthesis by M. J. Gait Download PDF EPUB FB2

A free online book on the chemistry and biology of nucleic acids, written by Prof. Tom Brown and Dr Tom Brown (Jnr). Call us: +44 (0)23 / Request a quote.

oligonucleotide synthesis Download oligonucleotide synthesis or read online books in PDF, EPUB, Tuebl, and Mobi Format.

Click Download or Read Online button to get oligonucleotide synthesis book now. This site is like a library, Use search box in the widget to get ebook that you want. A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology.

Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer.

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell Author: various.

Oligonucleotide Synthesis book Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology.

The most commonly used method for oligonucleotide synthesis is the cyclical four-step phosphoramidite synthesis method developed in the s (Caruthers et al.,; Fig. DNA oligonucleotides are synthesized in a 3′–5′ manner by coupling acid-activated deoxynucleoside phosphoramidites to an initial deoxynucleoside attached.

Opposite of biosynthesis, chemical synthesis proceeds in the 3' → 5' Oligonucleotide Synthesis book according to the steps outlined in Figure 1. Note: this article focuses exclusively on DNA synthesis (while similar, there are differences with the chemical synthesis of RNA).

Figure 1. Summary of. Please use one of the following formats to cite this article in your essay, paper or report: APA. Mandal, Ananya. (, February 26). Oligonucleotide Synthesis.

Oligonucleotide Synthesis: A Practical Approach (The Practical Approach Series) Paperback – January 1, by M. Gait (Editor) See all formats and editions Hide other formats and editions. Price New from Used from Format: Paperback.

Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure ().The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence.

Whereas enzymes synthesize DNA and RNA in a 5' to 3' direction, chemical oligonucleotide. Oligonucleotide Synthesis book. Read reviews from world’s largest community for readers.

A collection of powerful new techniques for oligonucleotide synt Author: Piet Herdewijn. Medical Book Oligonucleotide Synthesis Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation.

The phosphoramidite method of DNA synthesis is currently considered as the standard synthesis method used in most automated synthesizers today. This method allows achieving the high coupling efficiencies needed to synthesize longer and longer oligonucleotides with low amounts of failure sequences.

Oligonucleotide synthesis: A practical approach. Edited by M. Gait, IRL Press Limited, Oxford, pp. xiii+, pricef, $ (The Americas and Japan only).Author: M.

Buck. The oligonucleotide chain assembly proceeds in the 3' to 5' direction by following a routine procedure referred to as a "synthetic cycle". Completion of a single synthetic cycle results in the addition of one nucleotide residue to the growing chain.

A less than % yield of each synthetic step and the occurrence of side reactions set practical. RNA Synthesis Using 2′-O-(Tert-Butyldimethyls ilyl) Protection 3. RNA Oligoonucleotide Synthesis via 5′-Silyl-2′-Orthoester Chemistry 4. Dimethylthiarum Disulfide: New Sulfur Transfer Reagent in Phosphorothioates Oligonucleotide Synthesis 5.

Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes 6.

Since the length of an oligonucleotide is directly proportional to its synthesis cost, and inversely proportional to its efficacy, or the ability of the oligo to produce a desired or intended result, the desired oligo length is usually only a fraction of the targeted sequence’s length.

This book presents the latest knowledge on a range of topics relating to the synthesis of therapeutic oligonucleotides with the aim of providing new insights and inspiring fresh ideas in nucleic acid chemistry that may ultimately lead to the discovery of more effective nucleic acid drugs.

The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.

Keywords Therapeutic oligonucleotide Chemical modification Sugar Modification Artificial Oligonucleotides RNA Synthesis Natural Oligonucleotides. Introduction to the Synthesis and APPENDIX 3C Purification of Oligonucleotides ments, and check the log prior to synthesis.

A computerized log book is especially useful and allows for an organized oligonucleotide nomenclature (e.g., R may refer to Rebecca’s mer, the.

It is a temporary blocking group that will be removed to start the synthesis. The newly free 5’ hydroxyl group will be attached to a polymer, which is in turn attached to a glass bead, to begin oligonucleotide synthesis. This reaction is an example of an acid-catalyzed synthesis of an ether between the 5’-hydroxyl group and the DMT group.Open Library is an open, editable library catalog, building towards a web page for every book ever published.

Oligonucleotide synthesis by Piet Herdewijn,Humana Press edition, in English Oligonucleotide synthesis ( edition) | Open LibraryPages: Currently, North America leads the global oligonucleotide synthesis market vis-à-vis value and will likely retain its leading position in the years to come on account of pharmaceutical companies.